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Two difficulties tend to shorten the life time of the analytical column. First, solutes that bind irreversibly to the stationary phase degrade the column’s performance by decreasing the level of stationary section accessible for effecting a separation. Second, particulate material injected Using the sample may perhaps clog the analytical column.T
A different process, mass spectrometry, has specific strengths around other procedures. Mass spectra might be acquired quickly; only smaller total (sub-μg) of sample is required for analysis, and the data furnished by the spectra is incredibly educational with the molecular construction. Mass spectrometry also has robust advantages of specificity